Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1

Obtain biological samples

1.1

Locate and read background information for site or project

1.2

Review work request to identify required samples and the procedures, materials and equipment involved

1.3

Identify hazards and workplace control measures associated with collecting the sample, preparation methods, reagents and equipment

1.4

Assemble required materials, reagents and equipment and check they are fit for purpose

1.5

Collect samples in accordance with legislative requirements, codes of practice and workplace procedures

1.6

Log samples using workplace procedures

1.7

Record sample description, compare with specification and note and report discrepancies

1.8

Ensure traceability from receipt of sample to reporting of results

2

Prepare samples and grow cultures

2.1

Use aseptic techniques to prepare media for microbiological analysis

2.2

Obtain samples for microbiological analysis

2.3

Identify contamination pathways for both samples and media

2.4

Perform simple chemical tests to identify morphology of environmentally significant bacteria

2.5

Use aseptic techniques to manipulate samples and bacterial cultures

2.6

Use standard techniques to grow cultures of environmentally significant microorganisms

2.7

Use disinfection and sterilisation to control the growth of microorganisms

3

Prepare microscope for use

3.1

Select microscope and attachments for examination and measurements

3.2

Check that all microscope components are clean and fit for purpose

3.3

Mount sample in accordance with specified method

3.4

Adjust settings and alignment of optical and mechanical components to optimise performance

4

Perform microscopic examination in accordance with test methods

4.1

Identify microorganisms, cell structures and components of animal and plant tissues in environmental samples

4.2

Measure organisms and structures

4.3

Enumerate microorganisms in environmental samples

5

Classify organisms

5.1

Classify organisms into hierarchical structures using appropriate keys

5.2

Record all observations and measurements used to determine classifications

6

Relate cell structures and their functions to environmental adaptation

6.1

Identify cell structures from examination of biological specimens

6.2

Associate biological functions with cell structures

6.3

Associate cell physiology and enzyme cycles to environmental adaptation

6.4

Identify important biochemical pathways associated with environmentally significant organisms

7

Maintain a safe work environment

7.1

Use personal protective clothing and safety equipment to ensure safety and minimise cross-contamination

7.2

Handle all samples and equipment in accordance with workplace safety procedures

7.3

Clean up spills using techniques to protect personnel, work area and environment

7.4

Minimise generation of waste and environmental impacts

7.5

Collect and dispose of all wastes safely

7.6

Report hazards and incidents to designated personnel using workplace procedures

8

Maintain workplace records

8.1

Record approved data into workplace system

8.2

Maintain confidentiality and security of workplace information and data